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resource source identifier antibodies ha tag c29f4 rabbit monoclonal antibody cell signaling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resource source identifier antibodies ha tag c29f4 rabbit monoclonal antibody cell signaling
    Resource Source Identifier Antibodies Ha Tag C29f4 Rabbit Monoclonal Antibody Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c29f4+ha+tag+antibody/pm41920739-171-2-11
    Average 86 stars, based on 1 article reviews
    resource source identifier antibodies ha tag c29f4 rabbit monoclonal antibody cell signaling - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Inhalable Nanofitin demonstrates high neutralization of SARS-CoV-2 virus via direct application in respiratory tract.
    Article Snippet: .. Then, 3-mm sections were cut and staining with the C29F4 HA-Tag antibody (Cell Signaling Technology) diluted at 1:1,000 followed by an incubation with a anti-rabbit PE donkey IgG (Biolegend) at 1:800. .. Microscopy analysis was performed on a Leica microscope.

    Article Title: Inhalable Nanofitin demonstrates high neutralization of SARS-CoV-2 virus via direct application in respiratory tract
    Article Snippet: .. Then, 3-mm sections were cut and staining with the C29F4 HA-Tag antibody (Cell Signaling Technology) diluted at 1:1,000 followed by an incubation with a anti-rabbit PE donkey IgG (Biolegend) at 1:800. .. Microscopy analysis was performed on a Leica microscope.

    Incubation:

    Article Title: Inhalable Nanofitin demonstrates high neutralization of SARS-CoV-2 virus via direct application in respiratory tract.
    Article Snippet: .. Then, 3-mm sections were cut and staining with the C29F4 HA-Tag antibody (Cell Signaling Technology) diluted at 1:1,000 followed by an incubation with a anti-rabbit PE donkey IgG (Biolegend) at 1:800. .. Microscopy analysis was performed on a Leica microscope.

    Article Title: Inhalable Nanofitin demonstrates high neutralization of SARS-CoV-2 virus via direct application in respiratory tract
    Article Snippet: .. Then, 3-mm sections were cut and staining with the C29F4 HA-Tag antibody (Cell Signaling Technology) diluted at 1:1,000 followed by an incubation with a anti-rabbit PE donkey IgG (Biolegend) at 1:800. .. Microscopy analysis was performed on a Leica microscope.



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    Confocal images of 7 dpf zebrafish eyes showing transverse retinal sections stained with <t>anti-HA</t> (red), BODIPY (magenta), DAPI (blue), and GFP (green). a A negative control, pcdh15b-ko larvae without any transgene, showed no HA signal. b HA-tagged full-length Pcdh15b in the pcdh15b-ko background showed a strong HA signal localized to the photoreceptor layer. c HA-tagged mini-V4 in the pcdh15b-ko background exhibited a similar localization pattern to full-length Pcdh15b, with enrichment in the photoreceptor layer. d In the pcdh15b-het background, mini-V4 also showed a robust HA signal in photoreceptors with no signs of toxicity. These data confirm that mini-V4 recapitulates the subcellular distribution of full-length Pcdh15b in the retina. Scale bars: 10 μm.
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    Image Search Results


    Confocal images of 7 dpf zebrafish eyes showing transverse retinal sections stained with anti-HA (red), BODIPY (magenta), DAPI (blue), and GFP (green). a A negative control, pcdh15b-ko larvae without any transgene, showed no HA signal. b HA-tagged full-length Pcdh15b in the pcdh15b-ko background showed a strong HA signal localized to the photoreceptor layer. c HA-tagged mini-V4 in the pcdh15b-ko background exhibited a similar localization pattern to full-length Pcdh15b, with enrichment in the photoreceptor layer. d In the pcdh15b-het background, mini-V4 also showed a robust HA signal in photoreceptors with no signs of toxicity. These data confirm that mini-V4 recapitulates the subcellular distribution of full-length Pcdh15b in the retina. Scale bars: 10 μm.

    Journal: bioRxiv

    Article Title: Mini-Pcdh15b Gene Therapy Rescues Visual Deficits in a Zebrafish Model of Usher Syndrome Type 1F

    doi: 10.1101/2025.11.05.686814

    Figure Lengend Snippet: Confocal images of 7 dpf zebrafish eyes showing transverse retinal sections stained with anti-HA (red), BODIPY (magenta), DAPI (blue), and GFP (green). a A negative control, pcdh15b-ko larvae without any transgene, showed no HA signal. b HA-tagged full-length Pcdh15b in the pcdh15b-ko background showed a strong HA signal localized to the photoreceptor layer. c HA-tagged mini-V4 in the pcdh15b-ko background exhibited a similar localization pattern to full-length Pcdh15b, with enrichment in the photoreceptor layer. d In the pcdh15b-het background, mini-V4 also showed a robust HA signal in photoreceptors with no signs of toxicity. These data confirm that mini-V4 recapitulates the subcellular distribution of full-length Pcdh15b in the retina. Scale bars: 10 μm.

    Article Snippet: For immunofluorescence labeling, the following primary and secondary antibodies were used: anti-PCDH15 antibody (R&D Systems, AF6729), rabbit anti-HA (C29F4) antibody (Cell Signaling Technology, 3724), mouse monoclonal anti-rhodopsin (1:500) (MilliporeSigma, MAB5316), donkey anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 594 (1:200) (Invitrogen, R37119), donkey anti-sheep IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen, A-11015), donkey anti-mouse IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen, A32766), donkey anti-mouse IgG conjugated to Alexa Fluor 405 (1:200) (Invitrogen, A48257), and donkey anti-rabbit IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen, A-21206).

    Techniques: Staining, Negative Control

    a Immunostaining of adult pcdh15b wild-type retina with anti-Pcdh15 (red) revealed strong labeling along the calyceal processes and OS membranes of photoreceptors. BODIPY (magenta) marks OS membranes, and DAPI (blue) labels nuclei. Left: merged channels; right: Pcdh15 channel only. b, c Expression patterns of HA-tagged mini-V4 ( b ) and full-length Pcdh15b transgenes ( c ) in adult pcdh15b-wt retina. b Top left: anti-HA staining (red) of HA.mini-V4; bottom left: merged view showing HA.mini-V4 (red), BODIPY (magenta), GFP (green) expressed from the transgene, and DAPI (blue). Right: higher magnification of boxed region highlighting HA.mini-V4 localization to calyceal processes and OS. c Top left: anti-HA staining (red) of HA.full-length-Pcdh15b; bottom left: merged view showing full-length Pcdh15b (red), BODIPY (magenta), GFP (green), and DAPI (blue). Right: higher magnification of boxed region showing full-length Pcdh15b localization similar to mini-V4. Both constructs targeted correctly to the calyceal processes and OS membranes, mirroring the endogenous Pcdh15b pattern, indicating that mini-V4 retains the proper subcellular localization signals required for function in mature photoreceptors. Scale bars: 10 μm.

    Journal: bioRxiv

    Article Title: Mini-Pcdh15b Gene Therapy Rescues Visual Deficits in a Zebrafish Model of Usher Syndrome Type 1F

    doi: 10.1101/2025.11.05.686814

    Figure Lengend Snippet: a Immunostaining of adult pcdh15b wild-type retina with anti-Pcdh15 (red) revealed strong labeling along the calyceal processes and OS membranes of photoreceptors. BODIPY (magenta) marks OS membranes, and DAPI (blue) labels nuclei. Left: merged channels; right: Pcdh15 channel only. b, c Expression patterns of HA-tagged mini-V4 ( b ) and full-length Pcdh15b transgenes ( c ) in adult pcdh15b-wt retina. b Top left: anti-HA staining (red) of HA.mini-V4; bottom left: merged view showing HA.mini-V4 (red), BODIPY (magenta), GFP (green) expressed from the transgene, and DAPI (blue). Right: higher magnification of boxed region highlighting HA.mini-V4 localization to calyceal processes and OS. c Top left: anti-HA staining (red) of HA.full-length-Pcdh15b; bottom left: merged view showing full-length Pcdh15b (red), BODIPY (magenta), GFP (green), and DAPI (blue). Right: higher magnification of boxed region showing full-length Pcdh15b localization similar to mini-V4. Both constructs targeted correctly to the calyceal processes and OS membranes, mirroring the endogenous Pcdh15b pattern, indicating that mini-V4 retains the proper subcellular localization signals required for function in mature photoreceptors. Scale bars: 10 μm.

    Article Snippet: For immunofluorescence labeling, the following primary and secondary antibodies were used: anti-PCDH15 antibody (R&D Systems, AF6729), rabbit anti-HA (C29F4) antibody (Cell Signaling Technology, 3724), mouse monoclonal anti-rhodopsin (1:500) (MilliporeSigma, MAB5316), donkey anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 594 (1:200) (Invitrogen, R37119), donkey anti-sheep IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen, A-11015), donkey anti-mouse IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen, A32766), donkey anti-mouse IgG conjugated to Alexa Fluor 405 (1:200) (Invitrogen, A48257), and donkey anti-rabbit IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen, A-21206).

    Techniques: Immunostaining, Labeling, Expressing, Staining, Construct